Search results for " illumination"
showing 10 items of 38 documents
Tunable-frequency three-dimensional structured illumination microscopy with reduced data-acquisition
2018
The performance of a tunable three-dimensional (3D) structured illumination microscope (SIM) system and its ability to provide simultaneously super-resolution (SR) and optical-sectioning (OS) capabilities are investigated. Numerical results show that the performance of our 3D-SIM system is comparable with the one provided by a three-wave interference SIM, while requiring 40% fewer images for the reconstruction and providing frequency tunability in a cost-effective implementation. The performance of the system has been validated experimentally with images from test samples, which were also imaged with a commercial SIM based on incoherent-grid projection for comparison. Restored images from d…
4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)
2015
AbstractIn the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination microscopy (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since the common sample mounting procedure, based on gel embedding, could interfere with the sample morphology. In this work we propose an inverted selective plane microscopy system (iSPIM), based on non-linear excitation, suitable for 3D tissue imaging. First, the iSPIM architecture provides flexibility on the sample mounting, getting rid of the gel-based mounting typical of conventional SPIM, permitting 3D imaging of…
Enhanced field-of-view integral imaging display using multi-Köhler illumination.
2014
A common drawback in 3D integral imaging displays is the appearance of pseudoimages beyond the viewing angle. These pseudoimages appear when the light rays coming from each elemental image are not passing through the corresponding microlens, and a set of barriers must be used to avoid this flipping effect. We present a pure optical arrangement based on Köhler illumination to generate these barriers thus avoiding the pseudoimages. The proposed system does not use additional lenses to project the elemental images, so no optical aberrations are introduced. As an added benefit, Köhler illumination provides a higher contrast 3D display. This work was supported in part by the Plan Nacional I + D …
Lattice Corneal Dystrophy Type 1
2014
PURPOSE To evaluate the question whether lattice corneal dystrophy type 1 (LCD1) is of epithelial or stromal origin. METHODS The landmark of advanced LCD1 shows central superficial haze and paracentral stromal lattice lines. In 16 eyes of 8 affected individuals of 2 families, a penetrating keratoplasty was performed. The follow-up was 8 to 16 years after penetrating keratoplasty. Slit-lamp documentation of the patients was evaluated in direct and indirect illumination with dilated pupil to assess the horizontal and vertical pattern of new deposits on the corneal graft. Three affected patients of 2 families are demonstrated in detail. A DNA analysis was performed. RESULTS Gene identification…
Illumination Correction on MR Images
2006
Objective. An important artifact corrupting Magnetic Resonance Images is the rf inhomogeneity, also called bias artifact. This anomaly produces an abnormal illumination fluctuation on the image, due to variations of the device magnetic field. This artifact is particularly strong on images acquired with a device specialized on upper and lower limbs due to their coil configuration. A method based on homomorphic filtering aimed to suppress this artifact was proposed by Guillemaud. This filter has two faults: it doesnt provide an indication about the cutoff frequency (cf) and introduces another illumination artifact on the edges of the foreground. This work is an improvement to this method because i…
Express RGB mapping of three to five skin chromophores
2017
Skin melanin, oxy- and deoxy-hemoglobin were snapshot-mapped under simultaneous 448-532-659 nm laser illumination by a smartphone RGB camera. Experimental prototypes for double-snapshot RGB mapping of four (melanin, bilirubin, oxy- and deoxy-hemoglobin) and five (melanin, bilirubin, lipids, oxy- and deoxy-hemoglobin) skin chromophores with reduced laser speckle artefacts have been developed and tested. A set of 405-448-532-659 nm lasers were used for four chromophores mapping, and a set of 405-448-532-659-842 nm lasers for five chromophores mapping. Clinical tests confirmed functionality of the developed devices.
Bias artifact suppression on MR volumes.
2007
RF-Inhomogeneity correction is a relevant research topic in the field of Magnetic Resonance Imaging (MRI). A volume corrupted by this artifact exhibits nonuni- form illumination both inside a single slice and between adjacent ones. In this work a bias correction technique is presented, which suppresses this artifact on MR vol- umes scanned from different body parts without any a-priori hypothesis on the artifact model. Theoretical foundations of the method are reported together with experimental results and a comparison is presented with both the 2D version of the algorithm and other techniques that are widely used in MRI literature.
Automatic fringe pattern enhancement using truly adaptive period-guided bidimensional empirical mode decomposition.
2020
Fringe patterns encode the information about the result of a measurement performed via widely used optical full-field testing methods, e.g., interferometry, digital holographic microscopy, moiré techniques, structured illumination etc. Affected by the optical setup, changing environment and the sample itself fringe patterns are often corrupted with substantial noise, strong and uneven background illumination and exhibit low contrast. Fringe pattern enhancement, i.e., noise minimization and background term removal, at the pre-processing stage prior to the phase map calculation (for the measurement result decoding) is therefore essential to minimize the jeopardizing effect the mentioned error…
Efficient Skin Detection under Severe Illumination Changes and Shadows
2011
International audience; This paper presents an efficient method for human skin color detection with a mobile platform. The proposed method is based on modeling the skin distribution in a log-chromaticity color space which shows good invariance properties to changing illumination. The method is easy to implement and can cope with the requirements of real-world tasks such as illumination variations, shadows and moving camera. Extensive experiments show the good performance of the proposed method and its robustness against abrupt changes of illumination and shadows.
Comparison of single-spot technique and RGB imaging for erythema index estimation
2016
A commercially available point measurement device, the Mexameter(®), and an experimental RGB imaging prototype device were used for erythema index estimation of 50 rosacea patients by analysing the level of skin redness on the forehead, both cheeks and both sides of a nose. Results are compared with Clinician's Erythema Assessment (CEA) values given by two dermatologists. The Mexameter uses 568 nm and 660 nm LEDs and a photodetector for estimation of erythema index, while the used prototype device acquired RGB images at 460 nm, 530 nm and 665 nm LED illumination. Several erythema index estimation algorithms were compared to determine which one gives the best contrast between increased eryth…